【文獻標題】Environmental concentrations of antibiotics impair zebrafish gut health

【作者】Li Zhou，Samwel Mchele Limbu，Meilin Shen，et.al

【作者單位】華東師范大學（East China Normal University）

【文獻中引用產(chǎn)品】

魚丙二醛（MDA）ELISA試劑盒

【關(guān)鍵詞】Antibiotic，Intestinal microbiota，Gut health，Zebrafish

【DOI】https://doi.org/10.1016/j.envpol.2017.12.073

【影響因子(IF)】5.98

【出版期刊】《Environmental Pollution》

【產(chǎn)品原文引用】

 Biochemical assays

In order to minimize bias, each treatment contained 30 fish for biochemical analysis. The whole gut contents of each fish were weighed ａnd homogenized with 9 vol (v/w) of 0.8% physiological saline. Then, the homogenate was centrifuged at 2500×g at 4℃ for 10 min ａnd the supernatant was collected for biochemical assays according to the manufacturer＇s instructions. Malondialdehyde (MDA) was measured using an enzyme-linked immune sorbent assay (ELISA) kit (Hengyuan, Shanghai). Superoxide dismutase (SOD), peroxidase (POD), reduced glutathione (GSH), acid phosphatase (ACP), ａnd alkaline phosphatase (AKP) were measured using related commercial assay kits (Nanjing Jiancheng Institute,China). Results were recorded on a microplate reader (Epoch, BioTek, USA).

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原創(chuàng)作者：上海恒遠生物科技有限公司